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| Introduction of Recombinant DNA into Host |
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| Earlier E. coli was used as a host for the recombinant DNA. Now Bacillus subtilis and yeast are also being used. The bacteria to be used as hosts should be without plasmids. Their wall is made permeable by treatment with calcium chloride or lysozyme. The recombinant DNA is added to the culture in which such bacteria are growing. The recombinant DNA is taken up by bacteria along with nutrients. It replicates whenever the bacterial cell divides and may express the information of both the parent DNAs. The hybrid cells formed in the above process may be considered a new species. The technique allows the cloning of multiple copies of the desired gene. |
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