The process of conversion of free nitrogen of the air to useful nitrates is termed as nitrogen fixation.
- Biological nitrogen fixation
When nitrogen fixation is brought about by micro-organisms the process is called biological nitrogen fixation.
Examples: Rhizobium, a nitrogen fixing bacteria present in the roots of leguminous plants, fixes atmospheric nitrogen to the soil. Azotobacter and Clostridium are other free-living nitrogen fixing bacteria.- Physical fixation


- By the addition of compost and fertilizers.
B) How does nitrogen enter the body of plants and animals?
- The nitrates in the soil are absorbed by plants as mineral salts. Plants convert the inorganic nitrates to organic proteins.
- The proteins from the plants enter the body of animals in form of food.
- When plants and animals die their bodies decompose. The organic proteins undergo a series of chemical changes, brought about by micro-organisms e.g. ammonifying bacteria, nitrosomonas, nitrosococcus. These changes convert the proteins back to inorganic nitrates.
- In humans and animals some proteins are broken down to ammonia and carbon dioxide, which forms urea and is excreted out as urine. Their waste matter is also treated by micro-organisms to convert it into inorganic nitrates.
D) How does nitrogen enter the atmosphere?
Certain bacteria found in the soil called denitrifying bacteria convert nitrates of the soil to free nitrogen. Although this decreases the fertility of the soil it is inevitable.

