Vehicle DNA
The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA. Two types of DNA are used as vehicles: plasmid DNA and bacteriophage DNA..
Bacteriophage DNA
The bacteriophages (or phages), are viruses that infect and kill bacteria. Their circular DNA is also suitable for use as a vehicle DNA..
Replication of DNA
The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During replication, the DNA molecule unwinds itself fr..
Random DNA
It refers to small fragments formed by breaking a chromosome of an organism with the help of restriction endonucleas..
Structure of DNA
The structure of the DNA molecule and the arrangement of nitrogenous bases in the two polynucleotide chains, is very well explained by the double helix model proposed by Watson and Crick in 1953. Watson and Crick According to the double helix model, DNA molecule shows the f..
The structure of the DNA molecule and the arrangement of nitrogenous bases in the two polynucleotide chains, is very well explained by the double helix model proposed by Watson and Crick in 1953. Watson and Crick According to the double helix model, DNA molecule shows the f..Synthetic DNA (sDNA)
It is the DNA synthesised with the help of DNA polymerases on the DNA template or from free deoxybironucleotides without a templat..
Formation of Recombinant DNA (Integration of passenger DNA to vehicle DNA)
The DNA to be used as passenger or vehicle is isolated from the cell by lysing it with a suitable enzyme. The DNA is then isolated from other cell contents by ultracentrifugation and purified. Both the passenger and the vehicle DNA's are subsequently cleaved by using t..
Artificial Synthesis of DNA on Template
In 1961, Kornberg and his coworkers synthesized DNA from a mixture of deoxyribonucleoside triphosphates, DNA polymerase enzyme, metal ions and a segment of viral DNA functioning as a primer. The synthetic DNA showed biological activity. When introdu..
Recombination of DNA Technology
Recombination of DNA Technology - It is the most widely used technique in genetic engineering. It involves the formation of recombinant DNA and its introduction into an appropriate host. Recombinant DNA is the DNA formed by combining DNAs from different organ..
Recombination of DNA Technology
It is the most widely used technique in genetic engineering. It involves the formation of recombinant DNA and its introduction into an appropriate host. Recombinant DNA is the DNA formed by combining DNAs from different organisms. The technique requires skilful handlin..
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