description of dna replication

Replication of DNA - The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During r..

The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During replication, the DNA molecu..

This is a process in which a single DNA molecule produces two identical daughter molecules of itself. This replication is a semi conservative process. The parent DNA has two complementary strands, which unwinds by breaking hydrogen bonds. The separated strand acts as ..

It is a complex process and involves the participation of over a dozen enzymes and other protein factors. The intertwined DNA starts separating from a particular point and this is known as the origin of replication..

The semi conservative DNA replication was confirmed by an elegant experiment conducted by M. Meselson and F. W. Stahl in 1958. They grew the bacterium Escherichia coli in a medium containing nitrogen salts labeled with the stable heavy isotope 1 5 N. 1 5 N was incorporated ..

Introduction of Recombinant DNA into Host - Earlier E. coli was used as a host for the recombinant DNA. Now Bacillus subtilis and yeast are also being used. The bacteria to be used as hosts should be without plasmids. Their wall is made permeable by treatment with calcium chlori..

Earlier E. coli was used as a host for the recombinant DNA. Now Bacillus subtilis and yeast are also being used. The bacteria to be used as hosts should be without plasmids. Their wall is made permeable by treatment with calcium chloride or lysozyme. The recombinant DNA is added..

. DNA fingerprints are so accurate that even full siblings are readily distinguished from one another. More than 99 percent of the DNA is exactly the same in all humans. But DNA fingerprinting focuses only on the part that tends to differ from one person to the other. ..

Leading Strand - The enzyme forms one new strand in a continuous stretch in the 5'-3' direction and is called leading strand. On the other parent strand, the enzyme forms short stretches of DNA once again in the 5'-3' direction starting from an RNA primer. These short DNA fragme..

There is no interphase preceding second meiotic division. There is a brief intervening period called interkinesis. During this period there may be synthesis of some reserve food and proteins. However, there is no replication of DNA prior to meiosis I..