dna dna hybridization

It is the DNA synthesised on RNA template with the help of the enzyme reverse transcriptase and necessary nucleotides. The DNA strand is isolated from the hybrid RNA-DNA complex by using alkaline phosphatase enzyme. A complementary DNA strand is th..

Earlier E. coli was used as a host for the recombinant DNA. Now Bacillus subtilis and yeast are also being used. The bacteria to be used as hosts should be without plasmids. Their wall is made permeable by treatment with calcium chloride or lysozyme. The recombinant DNA is..

The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA. Two types of DNA are used as vehicles: plasmid DNA and bacteriophage DNA..

The bacteriophages (or phages), are viruses that infect and kill bacteria. Their circular DNA is also suitable for use as a vehicle DNA..

The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During replication, the DNA molecule unwinds itself fr..

The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During replication, the DNA molecule unwinds itself fr..

The structure of the DNA molecule and the arrangement of nitrogenous bases in the two polynucleotide chains, is very well explained by the double helix model proposed by Watson and Crick in 1953. Watson and Crick According to the double helix model, DNA molecule shows the f..

It refers to small fragments formed by breaking a chromosome of an organism with the help of restriction endonucleas..

The DNA to be used as passenger or vehicle is isolated from the cell by lysing it with a suitable enzyme. The DNA is then isolated from other cell contents by ultracentrifugation and purified. Both the passenger and the vehicle DNA's are subsequently cleaved by using t..

Double helical structure exists in six different forms. They are A-DNA, B-DNA, C-DNA, D-DNA, E-DNA and Z-DNA. Among these only 3 forms of DNA are important. They are B-DNA, A-DNA and Z-DNA..