enzymes enzymes of biology


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Cellular Macromolecules Introduction
Introduction - The living cells contain specific macromolecules with a high molecular weight and a poor solubility. Generally in a cell, macromolecules are represented by polysaccharides, proteins, nucleic acids and enzymes. These compounds are formed by polymerisation of micromolecules s..
Gene Metabolism Relationship
Gene Metabolism Relationship - The concept that genes have the information to produce enzymes which are proteinaceous in nature was proved by George Beade and Edward Talium in the early 1940s with their work on the fungus Neurospora crassa. This mould can normally grow in a simple minimal..
Digestion in Amoeba
Digestion in amoeba is intracellular taking place within the cell. The food taken in remains in a food vacuole or gastric vacuole formed by the cell membrane and small part of the cytoplasm. The vacuoles are transported deeper into the cells by cytoplasmic movements. Here they fuse with lysosomes t..
Complementary DNA (cDNA)
It is the DNA synthesised on RNA template with the help of the enzyme reverse transcriptase and necessary nucleotides. The DNA strand is isolated from the hybrid RNA-DNA complex by using alkaline phosphatase enzyme. A complementary DNA strand is then synthesized on the isolated..
House Keeping Genes
All the genes do not play the same role, nor are all the genes active all the time. With regard to their role and activity, the genes are of two different types- Those genes are constantly transcribed and expressed in the cells because the products they code for are constantly needed for cellular a..
Leading Strand
On the other parent strand, the enzyme forms short stretches of DNA once again in the 5'-3' direction starting from an RNA primer. These short DNA fragments are referred to as Okazaki fragments and because this strand is synthesised and put together in small pieces it is referred to..
Photorespiration
It has been observed that a high concentration of oxygen, inhibits photosynthesis. This is due to the reason that the CO 2 fixing enzyme RUBP carboxylase not only accepts CO 2 but can also combine with O 2 . Since the reaction is an oxygenation reaction, the same enzyme ..
House Keeping Genes
House Keeping Genes - All the genes do not play the same role, nor are all the genes active all the time. With regard to their role and activity, the genes are of two different types- Those genes are constantly transcribed and expressed in the cells because the products they code for are constantly..
Charging of tRNA
equation of trna charging process It is the step in which the amino acid AMP-enzyme complex joins with the amino acid binding site of its specific tRNA, where its COOH group bonds with the OH group of the terminal base triplet CCA. The reaction is catalyzed by the same enzyme, aminoacyl tRNA synthetase. The resu..
Mechanism of DNA Replication
Mechanism of DNA Replication - It is a complex process and involves the participation of over a dozen enzymes and other protein factors. The intertwined DNA starts separating from a particular point and this is known as the origin of replication. The second step is that the two strands of..
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