Prokaryotic Chromosome
Prokaryotic cells lack nuclear membrane and the genetic material is found in a compact region called nucleoid. The chromosome is made up of DNA and has associated proteins. It is about 1.2 mm in length in the bacterium Escherichia coli. The chromosome is highly coiled and super ..
Prokaryotic and Eukaryotic Cells
There are two basic types of cells found in living organisms - the prokaryotic cells and the eukaryotic cells. This classification is based on three main features, namely; Organisation of biomembranes Nature of cytoplasmic organelles and Arrangement of the nuclear material The prokaryotic cells do ..
There are two basic types of cells found in living organisms - the prokaryotic cells and the eukaryotic cells. This classification is based on three main features, namely; Organisation of biomembranes Nature of cytoplasmic organelles and Arrangement of the nuclear material The prokaryotic cells do ..Summary
) Auto analysers are Biomedical instruments, which are computer controlled and temperature regulated. 12) ELISA technique is widely used for the detection of virus, fungi and bacteria, It is used to detect infectious diseases like Tuberculosis and AIDS. 13) Invasive procedures like Endosc..
Vehicle DNA
The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called vehicle DNA. Two types of DNA are used as vehicles: plasmid DNA and bacteriophage DNA..
Bacteriophage DNA
The bacteriophages (or phages), are viruses that infect and kill bacteria. Their circular DNA is also suitable for use as a vehicle DNA..
Replication of DNA
The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divides. During replication, the DNA molecule unwinds itself fr..
Replication of DNA
The most significant feature of DNA molecule is its capacity to make its own identical copies. This property is called replication. It occurs regularly during the synthetic phase of the cell cycle, every time a cell divide..
Functions of DNA
DNA serves the following functions. It is the genetic material in all prokaryotes and eukaryotes. It is capable of replication by which it can be faithfully passed on to successive generations. It is involved in the synthesis of RNA. It provides the code for protein biosynthes..
Synthetic DNA (sDNA)
It is the DNA synthesised with the help of DNA polymerases on the DNA template or from free deoxybironucleotides without a templat..
Formation of Recombinant DNA (Integration of passenger DNA to vehicle DNA)
The DNA to be used as passenger or vehicle is isolated from the cell by lysing it with a suitable enzyme. The DNA is then isolated from other cell contents by ultracentrifugation and purified. Both the passenger and the vehicle DNA's are subsequently cleaved by using t..
Result
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