Enzymes
Enzymes - Enzymes are the biocatalysts of life. They are defined as biocatalysts synthesized by living cells. They are simple or conjugate protein and specific in action. At present about 300 enzymes are recognized and classified into six classes by International Union..
Properties of Enzymes
>o C. Enzyme activity decreases below and above this range of temperature. Each enzyme requires a specific pH for its activity. Enzyme activity decrease below and above this optimum pH. Enzymes generally work in groups ..
Activity - I
Take a thin layer of tissue from the lower surface of the leaf. Mount it on a slide in water and examine under a microscope. You will find tiny pores or stomata along with the epidermal cells. The stomata are bound by a pair of guard cells whose concave sides face each other. It is through the stom..
Take a thin layer of tissue from the lower surface of the leaf. Mount it on a slide in water and examine under a microscope. You will find tiny pores or stomata along with the epidermal cells. The stomata are bound by a pair of guard cells whose concave sides face each other. It is through the stom..Activity I
Hold a pin erect on a pile of papers. Keep another pin next to it upside down in such a way that its flat head rests on the pile. Press both these pins down by placing a flat object such as duster. We observe that pin which is erect pierces through the pile of paper..
Hold a pin erect on a pile of papers. Keep another pin next to it upside down in such a way that its flat head rests on the pile. Press both these pins down by placing a flat object such as duster. We observe that pin which is erect pierces through the pile of paper..Activity
Take a nichrome wire of about 50 cm length and apply a potential difference of 1.5 V from a battery. Measure the current flowing through the wire using an ammeter connected to it in series, and note the potential difference across the resistance using a voltmeter connected across it. Note down and..
Enzyme Catalysis
The remarkable specificity of enzymes comes from the ability of the enzymes to bring the substrates into a favorable orientation in the enzyme-substrate (ES) complexes such that the substrates are bound to specific regions of the enzymes. Each enzyme..
Classification of Enzymes
Enzymes are generally classified on the basis of the type of reactions that they catalyse. 6 groups of enzymes can be recognised on this basis. The following table lists the 6 groups of enzymes along with example. fig. 14.15 - Classification of Enzymes..
Enzymes are generally classified on the basis of the type of reactions that they catalyse. 6 groups of enzymes can be recognised on this basis. The following table lists the 6 groups of enzymes along with example. fig. 14.15 - Classification of Enzymes..Cleaving Enzymes
These are used to break DNA molecule into fragments. They are further of three kinds, Exonucleases, which release nucleotides from 5 or 3 ends of a DNA molecule; Endonucleases, which cleave DNA helix at any point except the at the terminal ends and Restriction endonucleases, which cleave D..
Enzymes and Inorganic Catalysts
Enzymes and Inorganic Catalysts - Enzymes resemble inorganic catalysts in several aspects and differ from them in many other features. These similarities and differences are summarised in the following table. fig. 14.12 - Similarities and Differences between Enzymes an..
Enzymes and Inorganic Catalysts - Enzymes resemble inorganic catalysts in several aspects and differ from them in many other features. These similarities and differences are summarised in the following table. fig. 14.12 - Similarities and Differences between Enzymes an..Activation of Amino Acids
It is the step in which each of the participating amino acid reacts with ATP to form amino acid AMP complex and pyrophosphate. The reaction is catalyzed by a specific amino acid activating enzyme called aminoacyl-tRNA synthetase in the presence of Mg2+. There is a separate amin..
It is the step in which each of the participating amino acid reacts with ATP to form amino acid AMP complex and pyrophosphate. The reaction is catalyzed by a specific amino acid activating enzyme called aminoacyl-tRNA synthetase in the presence of Mg2+. There is a separate amin.. Result
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