protoplast

Protoplast Culture - Protoplast is a cell without cell wall. In plants, the cells are invariably surrounded by thick cell wall made of cellulose and pectin. During the process of tissue culture and using it for crop improvement, cell wall acts as a barrier to induce mutations or..

Protoplast is a cell without cell wall. In plants, the cells are invariably surrounded by thick cell wall made of cellulose and pectin. During the process of tissue culture and using it for crop improvement, cell wall acts as a barrier to induce mutations or some times to develop th..

Each pollen grain is a haploid, unicellular mass of protoplast with a single nucleus. It is surrounded by a thick wall differentiated into two layers; the outer thick exine and the inner thin intine . The pollen grains reveal a wide range of microsculpturing of the exine under a scanning ..

Plasmolysis - If a plant cell is placed in a highly concentrated sugar or salt solution (i.e., hypertonic solution), water from the cell sap flows out due to exosmosis through the plasma membrane outside the cell. The loss of water from the cell sap causes contraction or shrinkage of the proto..

) which is activated to grow into separate organism by suitable mixture of cytokinins and auxins. The success in tissue culture depends upon a) the precision and length of asceptic condition b) source of explants c) compositions and concentrations of nutrients media d) time and dosage of hormones s..

>The individual fungal cells are bound by definite cell wall made up of chitin or cellulose or both. Chitin also called as fungal cellulose, is normally found in association with other compounds like proteins and lipids. The cell wall encloses protoplasts which are differentiated into pla..