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Enzymes
Several kinds of specific enzymes are employed in genetic engineering. These include lysing enzymes, cleaving enzymes, synthesizing enzymes, joining enzymes and alkaline phosphates..
Nucleotides
. Similarly, nucleosides involving deoxyribose sugars are called deoxy ribonucleosides. A nucleoside combines with a phosphate group to form a compound called nucleotide. Nucleotides formed by ribonucleosides are called ribonucleotides. They form the monomers of ribose..
. Similarly, nucleosides involving deoxyribose sugars are called deoxy ribonucleosides. A nucleoside combines with a phosphate group to form a compound called nucleotide. Nucleotides formed by ribonucleosides are called ribonucleotides. They form the monomers of ribose..Alkaline Phosphotase
These enzymes release phosphate group from the 5 end of linearised circular DNA to check its recircularizatio..
Summary
>Peptiglycons are found in the cell walls of prokaryotic cells. Proteins are polymers of aminoacids arranged in a specific sequence which is decided by the genetic code present in the DNA molecule. The polypeptide chain in a protein may be straight (primary structure) or may form helical coils (sec..
i) Binding to the receptor
Binding of insulin to the outer subunits of the receptor causes a conformational change in the membrane spanning -subunits, which is also an enzyme, a tyrosine kinase. The activated -subunits add phosphate groups of specific tyrosine residues located in cytoplasmic do..
Conversion to Ribonucleoside Monophosphates
The various ribonucleoside triphosphates on linking to the DNA template chain break off their high-energy bonds. This changes them to ribonucleoside monophosphates which represent the normal components of RNA, and sets free pyrophosphate groups (P~P). Pyrophosphate contains a high-energy ..
Charging of tRNA
It is the step in which the amino acid AMP-enzyme complex joins with the amino acid binding site of its specific tRNA, where its COOH group bonds with the OH group of the terminal base triplet CCA. The reaction is catalyzed by the same enzyme, amino..
Summary
>Steroids are lipids containing fused carbon rings. The most common steroids are sterols such as cholesterol and ergosterol. Amino acids are compounds containing a carboxyl (-COOH) and an amino (-NH 2 ) groups. There are about 28 amino acids available in cells of which 20 form the compone..
Summary
>The RNA nucleotides become linked with one another, resulting in the formation of a mRNA molecule. The process is catalysed by the enzyme RNA polymerase. The mRNA subsequently detaches from the DNA template and gets released. It comes out of the nucleus and reaches the cytoplasm. The sep..
Nucleotides
Base + Sugar + Phosphate = Nucleotide. When phosphate group is added to the above nucleosides then the nucleotide is formed. When a single phosphate group is added to the nucleoside then it is called as mononucleotide. The carbons of sugars are rep..
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